Find the nearest longines boutiques and retailers wustermark, germany. The cellometer has made it much easier to get cell numbers and viability percentages for use in downstream applications such as ivs and elispot. Cellometer auto 2000 comes with a starter set of 75 slides. I like the cellometer auto 2000 because it eliminates manual counting and. Cellometer auto 2000 user manual 2011 nexcelom bioscience llc cellometer auto 2000 user manual introduction 5 what is cellometer auto 2000.
At the completion of the oq the leco service engineer will provide instrument familiarization training and. Counting 1 x 10 6 cells takes approximately 5 minutes with a manual hemacytometer. Detailed description manuals and msds compatible instruments. The cellometer auto 2000 offers an allinone automated cell counter with an. The range of concentration that can be measured on the instrument is dependent on the size of the cell being counted i. Typically in less than 30 seconds per sample, cellometer cell counters provide cell sample analysis results that include cell count, concentration, diameter, images, and trypan blue viability. Cellometer auto 2000 for primary cell viability using dual fluorescence description the cellometer auto 2000 offers an allinone automated cell counter with an integrated 10.
The cellometer auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for. The cellometer auto 2000 automated cell counter provides an allinone touchscreen solution for pbmc counting while the cellometer k2 is optimized for complex primary samples such as hepatocytes. Cellometer auto 2000 cell viability counter from nexcelom. Comparing manual and automated cell counting methods. Automated cell counters with fixed single or dual fluorescent optics modules to determine viability while automatically excluding debris. As our channel brand, vwr offers an integrated, seamless purchasing experience.
Image cytometry with bright field plus 2 to 4 fluorescent channels for simple cellbased assays with flowlike data output. Ideal for direct manual addition and automatic dosing. The cellometer auto 2000 cell viability counter from nexcelom bioscience is a touchscreen, fluorescent automated instrument specifically designed for primary samples. The cellometer x1 and x2 automated cell counters have higher magnification for use with small cells such as yeast and platelets. The concentration can be measured from 1 x 105 1 x 107 cells ml without further dilution. Recommendedprocedureforcomparisonofcellconcentration. Nexcelom cellometer auto 2000 cell viability counter nexcelom cellometer auto 2000 cell viability counter the auto 2000 utilizes bright field imaging and dualfluorescence imaging to quickly and accurately identify and count individual cells.
Count peripheral blood mononuclear cells pbmc using the. In less than 30 seconds, cellometer automated cell counters eliminate tedious manual cell counting, improve data quality, and provide additional data like cell sizes, and cell images. This website uses cookies to make the content more userfriendly and effective. This page details the capabilities of the cellometer auto 2000, a cell viability. Cellometer auto 2000 cell viability counter nexcelom. Cellometer auto 2000 cell viability counter optimized. By using this website, you agree to the use of cookies.
Cellometer auto 2000 instrument with analyzing software 2. Hemacytometer, automated cell counters, cell analysis, cell counting. Cellometer auto t4 integrated scientific solutions. Fluorescent viability cell counters nexcelom bioscience. The company operates in more than 30 countries and delivers an extensive portfolio of products and services. The cellometer auto 2000 cell viability counter is a touchscreen. Cellometer auto 2000 cell viability counter braubeviale. The cellometer auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas. Auto 2000 viability of primary cells in complex samples containing debris and red blood cells.
Cellometer auto 2000 offers accurate and reproducible counting of peripheral blood mononuclear cells pbmc. The cellometer auto 2000 saves us so much time in daily lab work by eliminating manual cell counts after each passage or thawing. Load sample, view image, count cells, and obtain results in less than 30 seconds. The cellometer auto t4, a trypan blue viability cell counter, offers unique and superior functionalities and capabilities due to innovative design and sophisticated software. The precision of the auto 2000 counting method was determined by comparing the calculated % cv intra single user auto 2000.
The cellometer auto 2000 features onetouch assays for analysis of a wide range of primary samples, including. Accuracy of the cellometer auto 2000 counting method was determined by comparing the calculated % cv of two individuals. Installation qualification and operational qualification. Dualcolor fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Pipette 20ul of cell sample into one of the ports with any standard single channel pipette. The cellometer auto 2000 cell viability counter calculates cell count and concentration for live and dead cells and % viability in just 30 seconds. Disposable counting chambers disposable counting chamber accommodates 2 individual samples and can be loaded through either port. The cellometer auto t4 cell counter detects cells based on morphology, brightness, and size, and therefore, cellular debris is effortlessly and precisely eliminated from counting results. Automated cell counters and image cytometers nexcelom. The auto 2000 accurately calculates cell count, size, and viability measurements 10x faster than manual counting. Cellometer auto 2000, auto x4, x2, k2, vision cba and celigo. Compatible with cellometer auto 2000, auto x4, x2, k2, vision cba and celigo manual.
Pbmcs on a coulter counter, and once that broke, a manual hemocytometer. Cellometer auto 2000 integrated scientific solutions. Counting 1 x 106 cells takes approximately 5 minutes with a manual. Cellometer automated cell counters eliminate manual cell counting and deliver more accurate, consistent results in a fraction of the time. Product warranty is valid until expiration date stated on the product label.
Peripheral blood mononuclear cells are used to measure immunological functions. This procedure applies to the human papillomavirus hpv serology laboratory located at the advanced technology research facility atrf, room c2007. I like the cellometer auto 2000 because it eliminates manual counting and our counts are consistent between users. Serial twofold dilutions of rehydrated yeast were counted on the cellometer auto x4 10x to determine the linear range for reliable yeast concentration measurements. Perform twocolor fluorescence to count live and dead primary cells from peripheral blood, cord blood, and bone marrow. If no expiration date is listed, the warranty is valid for 12 months from the date of receipt of the product. Accurately measure pbmcs, stem cells, splenocytes, tumor lysates, and nucleated cells in samples containing debris and red blood cells in 30 seconds using samplespecific assays and a simple touch screen. Nexcelom cellometer auto 2000 primary cells youtube. Cellometer auto 2000 for primary cell viability using. By using advanced cell counting chamber technology, cellometer auto t4 eliminates cross contamination, allows for no maintenance and immediate setup and use.
If no expiration date is listed, warranty is valid for 12 months from the date of receipt of the product. Yeast counters by nexcelom is exhibiting the product cellometer auto 2000 cell viability counter at braubeviale 2019 in nuremberg germany. Dualcolor fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris. It takes about 5 minutes to count 1 x 106 cells with a manual hemacytometer. We count cells from primary samples and i like that the rbcs are not counted when we use the aopi o zhpu 4vmahujlyluly. Used nexcelom cellometer auto 2000 cell profiler 8004243.
Cellometer automated cell counters eliminate the drudgery of manual cell. This data set was taken on a concentration series of primary mouse splenocytes. Cellometer auto 2000 cell viability counter esbe scientific. The auto 2000 utilizes bright field imaging and dualfluorescence imaging to quickly and accurately identify and count individual cells. The cellometer %cv coefficient of variation, including sample preparation, was well under 5% for most cell lines tested.
Nucleated cells from whole blood pbmcs splenocytes following separation stem cells tumor and tissue cell suspension. Cellometer auto 2000 is an automatic cell viability counter with dual fluorescence. Since then, nexcelom has expanded our product range to. Cellometer auto 2000 for primary cell viability using dual. Cell count, concentration, diameter, and % viability are automatically calculated and reported. Cellometer auto 2000, x2, k2, vision cba and celigo manual. Cellometer k2 image cytometer for primary cells duration. Yeast analysis for breweries cellometer breweries and. The cellometer auto 2000 cell viability counter is a touchscreen fluorescent automated cell counter for primary samples. The cellometer auto t4 cell counter calculates cell count and concentration for live and dead cells and % viability in just 10 seconds. The cellometer auto 2000 offers an allinone automated cell counter with an integrated 10. Cellometer selection guide thermo fisher scientific. Cellometer auto 2000 cell analyzer demonstration thermolabmex. It is consistent, reliable, and accurate for primary cells, and no lysing of rbc needed.
The nexcelom cellometer auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex. Cellometer auto 2000 cell viability counter ae bios llc. Cellometer auto t4 automated cell counter offers signifcant advantages vs. Detect metabolically active calceinam positive cells and dead pi positive cells. Enables the users to detect metabolically active, calceinam positive cells. Counting live and dead cells sometimes takes twice as long.
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